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yacO [2019-06-28 09:32:36]
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yacO [2019-06-28 09:32:36]

putative 23S rRNA 2'-O-ribose G2251 methyltransferase
locus
BSU00960
pI
8.63
mw
27.36 kDa
protein length
249 aa Sequence Blast
gene length
750 bp Sequence Blast
function
rRNA modification
product
putative 23S rRNA methyltransferase
essential
no
ec
2.1.1.-
synonyms
rlmB
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.2|rRNA modification and maturation]

    • Gene

    Coordinates
    115,269 116,018

    The protein

    Protein family

  • [SW|Methyltransferase superfamily] (according to UniProt)

    • Paralogous protein(s)

  • [protein|8A2898B80D0FFA56C5AA86C6F09D886A62AABBDA|YsgA]

    • [SW|Cofactors]

  • SAM (according to UniProt)

    • Structure

  • [PDB|1GZ0] (from ''E. coli'', 44% identity) [Pubmed|12377117]

    • [SW|Localization]

  • cytoplasm (according to UniProt)

    • Expression and Regulation

    Operons

    genes
    [gene|132E91DB8C81CD8C4F5160B2C068B74DA10AE224|gltX]-[gene|5B9D5DA130DC3654F386684156BDC350DD05DB60|cysE]-[gene|E5826A99769E2FE45959A9D623D6467A8DDDEB81|cysS]-[gene|031FDF8443A8A4D5B042B7591EBE2E6C74F07702|mrnC]-[gene|8CB595F84D00D1262EB13E6CF47BE6F00EDE16A5|yacO]-[gene|172652156ABFE72D5BAAC22729CE17CA947538B5|yacP]
    description
    [Pubmed|7510287]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|7510287], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [regulon|T-box|T-box]: transcription antitermination, overlaps a transcription terminator upstream of [gene|5B9D5DA130DC3654F386684156BDC350DD05DB60|cysE], in [regulon|T-box|T-box]

    • regulation

  • expression transiently increases in the forespore [Pubmed|22848659]
  • the [SW|T-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-B884 (yacO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1883 NBRP B. subtilis, Japan]
  • BKE00960 ([gene|8CB595F84D00D1262EB13E6CF47BE6F00EDE16A5|yacO]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00960 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTGCATTTTTCCCTATGA, downstream forward: _UP4_AACCCTGTGGGAGAATAAAG
  • BKK00960 ([gene|8CB595F84D00D1262EB13E6CF47BE6F00EDE16A5|yacO]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00960 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTGCATTTTTCCCTATGA, downstream forward: _UP4_AACCCTGTGGGAGAATAAAG

    • References

  • 19258532,7510287,12377117,11698387